Principles of Psychobiology from Red Reef Publications
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Biological Bases of Mood Disorders 

· Are Mood Disorders Strictly Related to a Neurochemical "Imbalance"?
·Serotonin...The Master Mood Modulator
·Lithium and Bipolar Disorders
·Brain Circuits and Hallucinations
·The Amygdala and Fear
·More on the Cellular Differences in Depression
Recovery of Function and CNS Disease Processes

·Gene's Role in Parkinson's Disease may be Minimal
·More Attempts at Treating Parkinson's Disease
·Alzheimer's Disease: The Wicked Web
·More About Microbes and Alzheimer's Disease
·Establishing Functional Connections Following Spinal Cord Damage
·Brain Implants That Allow "Willful Thinking"
·Life Among Sick Brain Cells
·Brain Antibodies Provide Clues to Tourette's Syndrome
·Recovery of Function Might Be Speeded By Direct Electrical Stimulation Of The Brain
·Fetal Cell Therapy Benefits Some Parkinson's Patients
Language and Other Information Processing

·Biological Basis for Dyslexia (?)
·More on Neurological Substrates of Dyslexia
·Is it Time Yet?
·What's the Critical Period for Language Acquisition?
·Was Einstein's Brain Different?
Learning and Memory

·The Role of Zinc in Neural Communication
·The Hippocampus and Cabbie's Brain
·The Strength of Memories
·What Can Amnesia Tell Us About Human Memory?
Functional Plasticity

·Borrowing from Peter to Pay Paul ... Functional Plasticity Demonstrated in the Auditory Neocortex
·Sensory Experience Alters The Development Of Both Sensory and Motor Areas of the Neocortex
·Reorganization of the Somatosensory Thalamocortical Relay
·Clues about Phantom Pain -- Functional Plasticity in the Somatosensory System
Behavioral Pharmacology

·A Receptor for Addiction?
Biological Bases of Sleep and Dreams

·The Paradox of Sleep
·Is it Time Yet?
Critical Issues in the Neurosciences

·Adult Human Brain Cells May Have More Than One Birthday
·What Type of Neural Activity Promotes Development of Connections?
·Nature-Nuture in Stem Cell DNA
·Molecules That Guide Nerve Cells During Development

 
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Are Mood Disorders Strictly Related to a Neurochemical "Imbalance"?
(Probably Not...)

    Neuroscientists have found a severe depletion of cells in the brains of people who died with depressive illness.  The finding is the among the first pieces of physical evidence which confirms that depression and manic depression may be triggered by a specific abnormality in the organization of brain cells.  The unusual finding is that this abnormal organization of brain cells relates to glial cells (Module 1; Principles of Psychobiology), not neurons.
    Dr. Wayne Drevets and colleagues, researchers at the University of Pittsburgh, studied brain tissue from seven people that were diagnosed with either depression or manic depression, and found that anywhere from 40 to 90 percent of glial cells were missing in the region examined.  The area of the brain analyzed was within the prefrontal cortex (Module 5; Principles of Psychobiology), called the anterior cingulate cortex.  In 1996, Drevets reported that there was a significant decrease in blood flow in this region of the neocortex in living patients with manic depression and depression.
    The frontal neocortex and anterior cingulate gyrus has been associated with emotional processing, specifically how a person decides whether a certain behavior, thought, or feeling, will be rewarding.  People with mania, for instance, can be impulsive.  One theory is that they don't know what effect their behavior may have on themselves or others.
    In addition to establishing a support network for neurons and blood vessels, and acting to encapsulate and degrade foreign and abnormal material in brain tissue,  glial cells provide a transport system for specific ions (Module 1-2; Principles of Psychobiology)  that are important in neuronal signaling, specifically potassium (K(+)).
    Drevets said that he and his colleagues were surprised when they found that these depressive conditions may be associated with an abnormally low number of glial cells.  In research, glial cells have taken a back seat to neurons.
    The study was done in collaboration with then doctoral student Dost Ongur and Professor Joseph L. Price of the Department of Anatomy and Neurobiology at Washington University, St. Louis.
    "We weren't even going to count the glial cells because we thought that the neurons themselves would be down in number," Ongur said.  "We were stunned," Drevets added.  "We think this is the single most important finding in mood disorders."
    Drevets also suspects that glial cell loss contributes to the loss of brain volume noted on brain scans, causing an abnormality in the brain's ability to process emotions.


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More Attempts at Treating Parkinson's Disease; The Plot Thickens...

    Parkinson's disease involves a loss of both dopamine-containing neurons in substantia nigra, and axons of the nigrostriatal pathway (Modules 5 & 6; Principles of Psychobiology). Patients afflicted by this disease exhibit problems in both voluntary and involuntary motor movement, and symptoms can include tremors, rigid limbs and inability to move.
    Various attempts have been made to circumvent functional loss that accompanies Parkinson's disease.  In many people with advanced Parkinsonism,  treatment with levodopa (l-dopa; precursor to dopamine neurotransmitter production; Module 6; Principles of Psychobiology) has been initiated in an attempt to elevate levels of dopamine released by surviving dopamine projection systems.  However, l-dopa helps only some patients some of the time.
    Experimental methods have been attempted to circumvent the symptoms of Parkinson's disease.  One attempt has been direct electrical stimulation of the substantia nigra, which provided equivocal results.
    The basal ganglia include a group of forebrain structures that are all known to be involved in movement functions.  The substantia nigra, as well as other structures, are included in the basal ganglia.  Interconnections between these structures, and other structures such as the thalamus and cerebellum, establish a so-called "motor loop" (Module 5; Principles of Psychobiology).   Therefore, it may be possible that functional losses associated with degeneration of the nigrostriatal system could be offset by altering another component of the "motor loop".
        Electrical stimulation of another component of the basal ganglia has been shown to greatly improve the ability of Parkinson's disease patients to perform motor responses. Patricia Limousin of Grenoble University and colleagues found that electrical stimulation of the subthalamic nucleus caused a 60 percent improvement in patient's motor skills ability.  These results are reported in the New England Journal of Medicine.
        When l-dopa therapy was used, electrical stimulation improved symptoms slightly and helped decrease the jerky  movements that are a side effect of the drug. The average dosage of l-dopa was cut in half, and other drugs were also reduced or eliminated.  These results further reinforce the notion that many brain "systems" work in parallel to provide complex behavioral functions.


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Adult Human Brain Cells May Have More Than One Birthday

    It is widely believed that adult human brain cells do not regenerate.  Most textbooks state that  humans  are born with as many neurons as they will ever have, and that as we progress through life we may lose many of these original neurons.  A recent study may seriously challenge this information.
     A study by Peter S. Ericksson and colleagues at the Sahlgrenska University Hospital in Goteborg, Sweden, indicates that neurons in the hippocampus of adult humans may undergo what is known as neurogenesis.  These results are to be published in the November 1 issue of the journal Nature Medicine.
    Ericksson and colleagues utilized techniques acquired while visiting the Salk Institute's Professor Fred H. Gage, who participated in the study.
    The study employed tissue from terminal cancer patients who had undergone a diagnostic procedure that labels cells which are in the process of dividing or replicating.  In this procedure bromodeoxyuridine (BrdU) is delivered to patients by injection, and the nucleic acids of cells which are dividing incorporate the BrdU.  Later, the BrdU is visualized by the use of immunohistological methods (Module 6; Principles of Psychobiology), by using an antibody to BrdU.  Only cells that are in the process of dividing will incorporate BrdU, and the BrdU labeling therefore serves as a "birthday marker" for newly formed cells.
    Dr. Gage, discussed these results in an interview with National Public Radio© (NPR) (REAL AUDIO(tm) INTERVIEW).  UniSci Science and Research News also quoted Dr. Gage as stating that "All of the patients showed evidence of recent cell division."  He also added that "It's interesting to note that this was not a particularly young or healthy group of people, so new cell growth may usually be even more prominent that we observed."
     Evidence of neurogenesis has been gathering for years in infrahuman species, and this study is very important because it shows that human brain cells may, indeed, have the ability to replicate.  It is now important to determine whether or not these regenerating cells establish appropriate connections in the brain, or whether they merely replicate and then die, without establishing connections.  This line of investigation has obvious implications for clinical attempts to circumvent functional loss that results from CNS damage.


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Brain Implants That Allow "Willful Thinking"

    Neuroscientists have implanted a device in the motor neocortex of two people that has allowed them to operate a computer display by "thinking" about it.  It has been known for many years that direct electrical stimulation of particular brain regions can elicit sensory experiences, memory recall, or motor responses.  However, unlike scenes from many (oftentimes bad) science fiction movies, it has always been unclear whether or not brain cell activity could be used to control external machines.
    Dr. Phillip R. Kennedy, a researcher who has worked with researchers at Georgia Institute of Technology and Emory University, developed an implant that can be used to detect that activity of neurons, and convey these signals to computers for further processing and control operations.  The small recording sensor is enclosed in a glass envelope and coated with nerve growth factors that allow neurons in the region of the implant to establish functional connections with the sensor.  Normally, when recording electrodes are implanted in brain tissue the region surrounding the electrode is enveloped by glial cells (Module 1; Principles of Psychobiology) that attempt to encapsulate the "foreign" material.  This electrically isolates the recording electrodes from small amplitude potentials that are conveyed by individual axons, dendrites or gap junctions (Modules 1-5; Principles of Psychobiology).  The key development is the application of nerve growth factors that apparently encourages the growth of functional connections to the recording electrode -- This formation of intact connections could be followed after implantation by a change in the pattern of electrical activity detected by the electrode.
    Surgeries on two patients were performed by Dr. Roy Bakay from Emory, who presented the findings at the Congress of Neurological Surgeons annual meeting in Seattle.  The electrodes were implanted in the motor cortex, near the arm/facial region (Module 7c; Principles of Psychobiology), and signals were routed to a computer that moved a cursor across a screen to an icon region.  Both patients were paralyzed and unable to move their limbs or speak.  The first patient, who had the implant for 2.5 months before dying from amyotrophic lateral sclerosis,  learned to control the signals in an "on-off manner" for seven days.  The second patient (J.R.), who suffered brain stem stroke after a heart attack, has had the implant for 6 months.  Initially, this patient had a problem stopping his brain's electrical activity, but researchers programmed a pause into the system so that whenever the cursor landed on an icon, it stopped.  Eventually, the patient was able to stop the cursor at an icon and click it to say a word or a phrase.
    "This will hopefully open up a whole new world for J.R.," Bakay said.  "He is learning and so are we."

More on this development from National Public Radio...
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Serotonin...The Master Mood Modulator...

    People who suffer from anorexia/bulimia may be born differences in brain chemistry that makes them prone to eating disorders.  It has been known for many years that the brains of adult bulimics contain abnormal levels of the classical neurotransmitter serotonin (5-HT) (Module 6; Principles of Psychobiology), a neurotransmitter that influences mood states, obsessive-compulsive behaviors, and aggression.
      It has not been known whether these abnormal levels of 5-HT relate to the eating disorder, or whether 5-HT levels are abnormal prior to the onset of the eating disorder.  A recent study by Dr. Walter Kaye, professor of psychiatry at the Western Psychiatric Institute in Pittsburgh, suggests that serotonin levels may be abnormal before adult bulimics begin the cycle of bingeing and purging.
     The study compared 31 healthy women with 30 women who once suffered from bulimia but had returned to normal eating habits for at least a year.  Serotonin levels were measured in spinal fluid, and Kaye found that recovered bulimics had abnormally high levels of serotonin. Also, recovered bulimics showed many of the symptoms associated with high levels of serotonin, including negative moods and obsessions. Because all of the women in the study had resumed healthy eating habits, the findings indicate that diet, alone, cannot account for the altered serotonin found in individuals who are exhibiting bulimia. Instead, high serotonin levels may be a genetic condition that leaves some people prone to the disorder, Kaye said. Previous studies of twins of bulimics and anorexics also have indicated that the eating disorders may be genetic, "so there's accumulating evidence here."
       Women with high serotonin levels may be prone to eating disorders in part because the elevated serotonin levels tend to make them depressed, anxious or obsessed with control, and starvation can lower the level of serotonin. Serotonin is a product of tryptophan, which comes from amino acids in food. "Their abnormal eating habits make them feel temporarily better," Kaye said.
        But the serotonin levels may then drop below normal, which can cause feelings of impulsiveness, disorder and anxiety, so the bulimic person may binge to bring the serotonin back up again.  "They start to chase something they never really can catch," Kaye said.
 

 More on serotonin systems and suicide from National Public Radio...
 Click Here For NPR© Link (REAL AUDIO (tm) INTERVIEW)


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Alzheimer's Disease: The Wicked Web...

Background from National Public Radio (1997)
Click Here for NPR© Link (REAL AUDIO (tm) INTERVIEW)

    It now seems that a relatively harmless protein in the brain is somehow induced to cause damage to neurons when it changes molecular shape. As it undergoes this change, the protein called amyloid beta begins forming abnormal strand-like structures that accumulate on neurons. The accumulation of amyloid beta then appears to induce another normal protein, called Tau, to form aggregates that eventually kill brain cells.
    The two proteins distribute abnormally in individuals with Alzheimer's disease (Module 5; Neocortex; Principles of Psychobiology), forming the "plaques and tangles" that have characterized the disease since it was first described in 1907 by Alois Alzheimer. However, until recently, scientists have not known how or why these proteins are involved in the disease, there has been no general consensus on the reasons that plaques and tangles are produced in Alzheimer's disease.
    Three studies published in the July 1997 issue of Nature Medicine added additional insight into these questions. To place the significance of these results in perspective, it is important to understand that researchers have been divided into two "camps" for the past 15-20 years. Amyloid substance has been visualized in neurons of Alzheimer's patients for many, many years. Thus, it was suggested that amyloid protein may build up excessively in cells during Alzheimer's disease. Other researchers suggested that a so-called Tau protein, similar to cytoskeletal proteins (Module 1; Principles of Psychobiology), forms abnormal cytoplasmic aggregates in neurons called "tangles", which eventually kill the cell.
    As is usually the case in science, it appears that both camps are right...
Bruce Yankner and colleagues at Children's Hospital in Boston studied the brains of primates, which are genetically related to humans, and exhibit similar responses to brain disease. Yankner took the same amount of amyloid beta found in a human brain "plaques" and injected it into the brains of an aged rhesus monkey, a young rhesus monkey and aging marmoset monkeys, which are genetically different from rhesus monkeys. Yankner found that injections of amyloid beta into the brain of aged rhesus monkeys caused the same damage found in the brains of people with Alzheimer's disease. It also caused changes in Tau protein, which appeared to begin forming tangles. The brain of the young rhesus monkey did not respond to the injection of amyloid beta, and the brains of aged marmosets were only slightly affected.
    The young rhesus monkey was apparently protected from developing plaques. This experimental observation is interesting because, on average, symptoms of Alzheimer's disease do not manifest until individuals are 60-65 years of age.
    A second study conducted by Claudio Soto of the New York University Medical Center. provides dramatic evidence that a drug may be developed to block amyloid protein from becoming harmful. The study also shows it may be possible to dissolve the plaques on brain cells after they have formed.
    Soto and colleagues conducted experiments with live rats and human cultured cells. They created a synthetic agent that binds to a part of the amyloid protein that is involved in the twisting and folding that occurs when normal amyloid changes to become toxic. When delivered to rats, the compound completely blocked the induction of plaques by injected amyloid. Moreover, the synthetic compound dissolved plaques on human brain cells that were grown in culture--it was as if the synthetic compound "unwound" the plaque. The synthetic agent is a peptide made up of only five amino acids.
    Finally, researchers at the Institute for Physical Biology in Dusseldorf, Germany, have developed a technique for diagnosing altered forms of amyloid in spinal fluid of patients with Alzheimer's disease.   This method employs fluorescence correlation spectroscopy to detect very small aggregations of amyloid in patients with the disease. The test detected these aggregations with nearly 100% accuracy.

We still are not sure what induces the development of altered amyloid protein, but the studies mentioned above begin to give us ways to possibly slow, or eliminate, the progression of the disease.
 


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Establishing Functional Connections Following Spinal Cord Damage...There May be Hope...

    Researchers at Purdue University have apparently confirmed that it is possible to "fuse" axons of the spinal cord that have been severed by trauma.  By performing studies on isolated spinal cords derived from guinea pigs the researchers have, for the first time, recorded electrical potentials  from a previously severed spinal cord axons. Not all of the axons were experimentally repaired following damage, but the fact that some axons were repaired is a great step forward.  "If you have even 5 percent of the nerve fibers carrying nerve impulses, you'll get significantly more than 5 percent back in terms of restored behavior," says Richard B. Borgens. He and his colleague, assistant professor Dr. Riyi Shi, both of Purdue's Center for Paralysis Research in the School of Veterinary Medicine, will report their findings today in Long Beach, Calif., at the 18th annual meeting of the Society for Physical Regulation in Biology and Medicine. Borgens also has submitted a paper on the research to the Journal of Neurotrauma.
    For many years researchers have known that certain chemicals, such as polyethylene glycol (PEG), have the ability to fuse lipid membranes.  In fact, PEG is used in the now-common technique of monoclonal antibody production to fuse different antibody-producing cells together.  In their spinal cord studies, Dr. Borgens used a similar approach to  fuse cut axons of the spinal cord.  Obviously, it is not possible to re-establish the original connections, but connections are established. Electrical impulses were restored in all of the guinea pig cords used in the study. The repaired spinal cords transmitted between 5 percent and 58 percent of pre-cut impulses.
    Borgens and Shi applied polyethylene glycol, or PEG -- a nontoxic, water-soluble polymer used in medicine and  cosmetics -- across the region of the guinea pig's spinal cord that had been severed but gently pressed back together. PEG was applied for two minutes, then removed. The polymer "fused" the membranes of a significant number of axons, apparently establishing physical connectivity with regions above and below the cut..
     Using an apparatus and procedure that Shi designed, the researchers then applied a small electrical current to one end of the cord to stimulate nerve impulses to travel towards the other end (Module 3; Principles of Psychobiology). Between five and 15 minutes after the PEG application, nerve fibers were repaired, allowing some impulses to reach the other end of the severed cord in 100 percent of the cases.  The authors also confirmed connectivity by the use of tract-tracing techniques (Module5: Principles of Psychobiology)
    Unisci News quoted the authors as stating that: "In most spinal cord injuries in animals and in people, the spinal cord is not completely severed, as we have done in these first experiments," he says. "The cord is more likely to be crushed, and the nerve fibers develop holes in their membranes, which ultimately leads to separation of the nerve fiber within 24 to 72 hours."  The PEG method may allow trauma care facilities to "plug-up" damaged membranes before neural degeneration begins.
    Borgens and his colleagues have done extensive work in producing other methods to treat naturally paralyzed dogs with spinal cord injuries, and some methods have moved to testing phases in human spinal cord injuries.


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Some Background (12/96) about the Memory of Mice with "Holes in Their Heads"...
Click Here For NPR© Link (REAL AUDIO (tm) INTERVIEW)

The Hippocampus and Cabbie's Brains
 

    For many years,  the hippocampus has been implicated in spatial memory functions in animals (Module 5; Principles of Psychobiology).  For instance, animals that have received experimental damage to the hippocampus exhibit problems utilizing spatial stimuli during learning tasks and/or they cannot remember spatial "maps" (such as maze routes) as well as normal animals.  The hippocampus in humans appears to function similarly.  National Public Radio conducted an interview in November 1997 with an investigator who used a cleaver method to investigate this spatial function in humans.

More on the Human Hippocampus from National Public Radio...
Click Here For NPR© Link (REAL AUDIO (tm) INTERVIEW)
 


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Biological Basis for Dyslexia (?)

        Dyslexia is a disorder in which individuals appear to process written words, and acoustical (mental) representations of written words, much differently that normal individuals.  For instance, dyslexics oftentimes have trouble learning words and reading. They may have trouble rhyming words, may mix up letters such as "d'' and ''b'', and may have trouble spelling -- despite often above-average intelligence and motivation.     Dyslexia tends to run in families, suggesting that there is a genetic basis for the symptoms.     There are no firm figures on the incidence of dyslexia in the population, but 10 to 15 percent of school children have learning disorders, and 80 to 85 percent of them have language and reading based problems.  The International Dyslexia Association suggests that a large proportion of such problems may relate to dyslexia.
      Researchers have found differences in the way that information is processed in the brains of dyslexics. Dr. Sally Shaywitz of Yale University and colleagues  used magnetic resonance imaging (MRI) to study the brains of 32 normal volunteers and 29 dyslexic adults.  The individuals in the study were asked to take a series of reading tasks.
     Shaywitz's team found that non-dyslexic people increased their brain activity as reading tasks got harder, while dyslexics did not.  When the dyslexics read, Shaywitz's MRIs recorded a pattern of under-activation in a large area at the back of the brain associated with language.   In particular, they found the angular gyrus, which is considered key to the visual and language skills needed for reading, was disrupted in the dyslexics. Wernicke's area, which is nearby and important to language comprehension, was also under-activated in dyslexics.
    ``These findings have important implications for the large numbers of intelligent men, women and children with dyslexia,''  the researchers, who reported their findings in the Proceedings of the National Academy of Sciences, said in a statement.     ``If you have a broken arm you can hold up an X-ray as evidence. Up to now, individuals with dyslexia were often doubted and there was little concrete evidence they could show to support the neurobiologic nature of their reading difficulty.''     Previously,  British researchers reported that they had found three common genes associated with dyslexia in blood samples from 90 families.
 

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View Quicktime Movie from Philadelphia Newspapers Inc. about results from studies of Dyslexia
 

More on Dyslexia from National Public Radio...
Click Here For NPR© Link (REAL AUDIO (tm) INTERVIEW 03/02/98)
 


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  The Paradox of Sleep

   We spend roughly 30% of our lives in sleep, and one of the most fascinating questions in the neurosciences has been "Why do we sleep, and what brain mechanisms cause sleep."  These are valid questions for the neurosciences, because certain brain injuries and abnormalities can dramatically alter the wake-sleep cycle (Module 5: Principles of Psychobiology) (see below).
    Although several theories have advanced to explain the why of sleep, it is generally believed that sleep serves to aid in some form of restorative function.  Key data that support these theories are related to the observation that sleep deprivation causes adverse physiological changes in animals and humans, sometimes resulting in death.
    Many of the classical neurotransmitters have been implicated in sleep.  It is generally thought that "excitatory" and "inhibitory" transmitters somehow interact to modify particular wake-sleep states.  Moreover, there may be a control mechanism of some sort that affects how particular transmitters function to produce wake-sleep states.
    In early 1996 an interesting hypothesis was presented which suggests that sleep does serve a restorative function with regard to energy restoration in brain cells.  Specifically, it is proposed that low levels of extracellular glucose trigger the release of the nucleotide adenosine, which then may serve to inhibit several of the "excitatory" neurotransmitter receptors.  During this process, glycogen stores in astrocytes (glial cells: Module 1: Principles of Psychobiology) also may be converted to glucose, thereby restoring extracellular energy reserves for neurons.  This proposal was presented by J.H. Benington and H.C. Heller in Progress in Neurobiology, and NPR conducted an interview with Dr. Heller, of Stanford  University about the energy-restoration hypothesis.

More on this Hypothesis from National Public Radio...
Click Here For NPR© Link (REAL AUDIO (tm) INTERVIEW)

    What about the where of sleep in the brain?  The reticular (activating) system, Raphe' nuclei, locus coeruleus, and hippocampus have been implicated as brain structures that regulate sleep (Module 5: Principles of Psychobiology).  In 1996, J.E. Sherin, P.J. Shiromani, R.W. McCarley, and C.B. Saper presented data in Science which may implicate a critical brain region that serves as a "switch" to control the activity of many of the aforementioned  nuclei.  Not surprisingly, this potential "sleep switch" may be located in the hypothalamus, together with other control centers for vital body functions.

More on a Potential "Sleep Switch" from Phillynews
Click Here for the Link to Phillynews Archives
 


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Lithium and Bipolar Disorders
 

    Nearly 50 years ago, Australian psychiatrist John F. Cade demonstrated that  lithium ions exert a mood-stabilizing effect on individuals with manic-depressive symptoms (bipolar disorder).  It has been estimated that approximately 2.5 million Americans may be afflicted with a bipolar disorder.  Since Cade's early observations, lithium has been termed a "wonder drug" in clinical treatment.  However, while it is generally assumed that that lithium somehow affects the CNS in individuals with bipolar disorders, the way in which lithium operates to stabilize mood swings has remained elusive. Researchers have new clues in the puzzle of how lithium can effectively stabilize bipolar disorders.
    A study reported in the July 7, 1998 Proceedings of the National Academy of Sciences found that, in mice, lithium exerts a push/pull effect on the synaptic levels of glutamate, eventually causing it to stabilize at a level where it can control both mood extremes.  University of Wisconsin Medical School professor of pharmacology, Dr. Lowell Hokin, directed the research.
    Glutamate is the primary excitatory neurotransmitter in the brain (Module 6; Principles of Psychobiology), and it has been estimated that approximately 85 percent of neurons in the brain either release glutamate OR are receptive to glutamate. Other neurotransmitters include serotonin, dopamine, norepinephrine and acetylcholine, but the glutamate system is extremely widespread in the brains of all organisms. Normally, presynaptic potentials cause a  release of glutamate which interacts with receptors postsynaptically. Re-uptake transporters located presynaptically then reabsorb some of the glutamate, pumping it back into the cell for reuse (Module 6; Principles of Psychobiology).
    If the reuptake mechanism malfunctions, inappropriate concentrations of glutamate can remain in the synapse. Hokin postulates that abnormally low glutamate levels are involved in depression, while high levels are responsible for mania.
    In an earlier study (reported in PNAS, Aug. 30, 1994), Hokin and colleague showed that lithium causes glutamate to accumulate in synapses of mice and monkey brain slices, but exactly how it worked remained unclear. In the current study, functioning slices of mice brain were examined following exposure to lithium, while control slices were not exposed to the drug. The researchers observed that lithium raised the synaptic glutamate level by slowing its reuptake. The higher the lithium dose, the greater the accumulation of glutamate.
    Clinically, it takes a while for lithium to exert its effects on bipolar disorders.  To experimentally study the chronic effect of the lithium, the UW team administered it to live mice for two weeks. To their surprise, they saw that glutamate reuptake increased. This "up-regulation" of transporters resulted in a lowering of synaptic glutamate, which would produce an anti-manic effect. "We were especially interested to find that the reuptake mechanism in the 18 lithium-treated mice was stabilized in a very narrow range, as compared to the 18 controls," he said.
    Hokin proposes that  a compensatory mechanism in the reuptake system strives, over time, to stabilize synaptic glutamate  in a fixed range. When the levels of glutamate are too low, as postulated in depression, lithium may elevate synaptic glutamate.
    The research findings support clinical observations, he noted. "It takes a few weeks before lithium begins to relieve depression and mania in bipolar patients," he said. "It's now apparent an adaptive reuptake mechanism that brings glutamate within a 'normal' range works over time to curb both the highs and lows."  Moreover,  he added, lithium doesn't change the moods of people who aren't bipolar, suggesting that their glutamate levels may be positioned consistently within the set zone, and therefore would not be affected by the drug.


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More About Microbes and Alzheimer's  Disease

    For about 15 years researchers that have examined the brains of Alzheimer's patients have noticed something very unusual.  In addition to the characteristic neuronal anomalies that characterize Alzheimer's disease, such as "plaques and tangles", investigators have been seeing what appears to be both bacterial and viral fragments in areas of the brain that have degenerated.  This has given rise to one hypothesis that Alzheimer's disease may have an infectious basis.  In fact, a correlation has already been established between late-onset Alzheimer's and infection with herpes simplex virus type 1.
    One group of neuroscientists recently identified a specific bacterium in the brains of Alzheimer's patients, and these results were presented at the 1998 annual meeting of the Society for Neuroscience.  These investigators examined brain tissue of Alzheimer's patients after autopsy and found that a bacterium called Chlamydia pneumoniae had invaded brain cells in regions where Alzheimer's damage was detected.  Researchers were surprised to find the presence of the bacterium because it normally cannot cross the blood-brain barrier, and in Alzheimer's patients it appeared to have traveled far beyond its common entry point into the body, the sinuses and lungs.
    The finding is important to "our potential understanding of how an infectious agent could lead to the neurodegeneration observed in Alzheimer's disease," according to Brian J. Balin, of Allegheny University of the Health Sciences in Philadelphia.
    Rather then observing what was suspected of being bacteria, Balin and colleagues analyzed DNA segments from the putative bacteria.  The bacterium type was confirmed in post-mortem brain samples of 17 of the 19 Alzheimer's patients he analyzed. Conversely, 18 of 19 brain samples from people that died of other causes showed no signs of the organism.
    "At this time, we do not know if the presence of this organism is causative or if it is a risk factor for the disease," Balin wrote. "However, we believe that our findings support those of others suggesting that inflammation, which can be stimulated by this bacterial infection, is a very important aspect of late-onset Alzheimer's disease."   When cells become infected with the bacterium, the cells produce chemicals to fight the infection. Some of these inflammatory chemicals, called cytokines, can damage nerve cells.
     This suggestion has good face validity because it has recently been reported that patients taking over-the-counter anti-inflammatory compounds, such as aspirin and ibuprofen, show a slower rate of disease progression.
    Zaven Khachaturian, a consultant to the Alzheimer's Association,  said the critical question is to identify the trigger, which he suggested might be some sort of trauma or other incident that creates damage to the blood-brain barrier and lets the bacteria in. "Now you have a break in the pipe...setting the stage for other events to occur. I think this is a likely scenario."
    Khachaturian said it's also important to find out whether the bacteria has set the stage for Alzheimer's in the patients who died, or whether the Alzheimer's damaged the brain, letting in the bacteria.
    Interestingly, the same bacterium has been detected in the fatty plaques that clog heart arteries, though researchers do not yet know if it is responsible for the clogging.


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  Brain Circuits and Hallucinations

    Neuroscientists have caught a glimpse of what appears to be brain circuits that are unusually active during schizophrenic hallucinations,  where patients hear voices and see things that aren't there. The observations may help researchers understand why some individuals "hear" or "see" something that is not "real".
     In the study, psychiatrist and neurologist David Silbersweig, MD, radiologist Emily Stern, MD, of Cornell University, and colleagues at MRC cyclotron facility, Hammersmith Hospital in London observed that  hallucinating patients share a similar pattern of activity in a set of interconnected regions deep in the brain. Silbersweig and colleagues used positron emission tomography (PET) to study six hallucinating schizophrenics. Participants were told to press buttons connected to the scanner whenever they heard voices talking to them or saw hallucinatory images.  Five of the participants were experiencing auditory hallucinations even though they were on medications, and one had both auditory and visual hallucinations and was not taking medication. The team was aided in the study by colleagues at Hammersmith Hospital in London.
    In the five patients experiencing auditory hallucinations, PET showed strong activity in systems associated with the frontal lobe, a neocortical area responsible for integrating current and past events, language, cognitive concepts of space and time, and emotions (Module 5: Principles of Psychobiology). The areas included the mediodorsal thalamic region, left hippocampus/parahippocampal gyri, right anterior cingulate neocortex and left orbitofrontal cortex. Many of these regions have been implicated by other research as being involved in schizophrenia.
    In addition, the single patient experiencing both auditory and visual hallucinations showed activity in the visual association cortex and the auditory association cortex. Most of the neocortical activity in this patient took place in the left hemisphere, where  language-related information would be integrated with visual information. When activity of neocortical structures was compared to the subcortical structures, activity in the subcortical brain regions was abnormally high.
    When "normal" people hear real voices or see real images, their brains show similar patterns of activation in the neocortex, but fewer of the subcortical brain regions are activated, Silbersweig said.  This work gives clues about where to look for a difference in the "hallucination circuitry" of different people.  For instance; very high levels of activity in subcortical areas of schizophrenics may indicate that the hallucinations are being "made" in those regions of the brain in the absence of "real" external auditory or visual stimuli. When the neocortex receives input from these abnormally active subcortical structures, it may not "test" the validity of the input:   The neocortex may simply  "create its own reality and believe it"  based solely on the type of neural input it receives.  These results were published in Nature.

   Here's a thought question:  Do you think that understanding these brain mechanisms may also tell us something about the nature of dreams or Visa Versa?
 


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  Last Update: 08/25/1999
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